Bauhinia vahlii is a medicinal plant with therapeutic potential, belonging to the family Fabaceae, and is also known as Thanjedu, recognized for its antibacterial activity. Recently, many biological activities of Bauhinia vahlii L. seeds have been reported, including traditionally, said to be medicinal because it can relieve inflammation irritation causes increased passing of urine and gives tonic effects and also cucurbit pepo (pumpkin), Bauhinia vahlii Colocynths (bitter cucumber), among from that I have been taken Bauhinia vahlii seed (Thanjedu) is one of most effective and potential anti-bacterial activity, particularly in its seed. The result of the antibacterial activity indicates that the effects of Thanjedu seed extract were tested on phytochemicals, and the results showed the presence of alkaloids, flavonoids, corticosteroids, saponins, and tannins in the extracts of Thanjedu. Among those results, alkaloids and flavonoids were the most effective compounds for the antibacterial activity in higher plants. Apart from that, saponins show the most responsible and effective compound for antibacterial activity of Thanjedu. In this study, two solvents, chloroform seed extracts and acetone seed extract from Thanjedu L., have been tested for Anti-bacterial activity. The antibacterial activity of the extract was examined against Gram-positive and Gram-negative bacteria by measuring the zone of inhibition. Results showed acetone extract was increased linearly increasing different concentration of extracts (25 mg/ml, 50 mg/ml, 75 mg/ml, 100 mg/ml) against two pathogenic strains (Escherichia coli, Staphylococcus aureus), by disc diffusion method, as compared with standard drug such as ciprofloxacin and control as acetone, the antibacterial activity of acetone was good. Both organisms used for screening have similar sensitivity.