This paper will explore biomechanical and anthropometric factors that determine the performance of motor fitness in the handball players of urban, semi and tribal areas. The comparative cross-sectional design was used in the study, which involved 90 participants whose age ranged between 18 and 25 years and was evenly distributed in the three categories. The anthropometric measurements involving height, weight, body mass index, arm span and leg length were discussed in association with biomechanical measurements of a jumping force, stride length and joint angles with motor fitness measures of speed, agility, strength, endurance and flexibility. The data indicate substantial differences based on the region and that the urban players have better anthropometric benefits and upper-body strength, whereas the tribal players were more successful in speed, agility, endurance, and flexibility. Semi-urban players were performing fairly generally in most of the variables. Correlation and regression models have revealed that jump force, stride length, height and arm span are the key predictors of the motor fitness performance, with a significant percentage of variance in the performance. The analysis points out that the structural attributes, as well as the movement efficiency, have a significant effect on athletic performance. Moreover, the existing differences between different regions depending on the availability of training, the essence of lifestyle and the environment have a very important impact on the achievement of fitness. In order to optimise performance and talent in handball, the results highlight the necessity of considering approach and longer region-based training interventions.