The purpose of the study was to assess the selected physiological variables on state level volleyball referees of Gujarat state. Gujarat state volleyball Association was conducted volleyball Referees examination in May 2012. 60 Candidates were selected to conduct the study from this examination, age ranging from 25-35 years. Selected physiological variables for the study were body mass index, basal Metabolic rate, fat percentage and lean body Mass. To calculator, Bmi and Bmr calculator software were used and also height, weight, age, gender and skin-fold measurements were taken with the help of instruments such as stadiometer, weighing machine and large skin fold caliper. Descriptive statistical analysis has been done by T-test and the significance of the result was seen on 0.05 levels. The t-test showed that significant difference was found for fat percentage among male and female volleyball referees of Gujarat state, whereas no significant difference was found for BMR, BMI and Lean body mass among male and female volleyball referees of Gujarat. The study revealed that female volleyball referees have higher percentage of fat than male volleyball referees of Gujarat.