The Gomti River originates from Gomati Taal (Fulhaar jheel) near Madho Tanda, Pilibhit, India, stretching over 960 km through Uttar Pradesh before merging with the Ganges near Saidpur, Kaithi, 27 km from Varanasi district. Considering global concerns about pollution, maintaining clean water, air, and soil is essential for human well-being. However, contemporary challenges include the inflow of untreated waste, agricultural runoff laden with pesticides and fertilizers, as well as pollutants such as oil, asphalt, sediment, and heavy metals into the Gomti River. This study focuses on a segment of the Gomti River within Lucknow city, Uttar Pradesh, spanning from 80.899893 to 80.968180 N latitude and 26.886799 to 26.833321 E longitude. Eight sampling sites were selected between Guaghat upstream and Piparaghat to conduct a comprehensive assessment of physio-chemical parameters. Water samples collected from these locations were analysed to gauge the river's water quality. Parameters including temperature, total suspended solids (TSS), total dissolved solids (TDS), pH, hardness, turbidity, dissolved oxygen (DO), nitrate, chlorine, alkalinity, calcium, magnesium, biochemical oxygen demand (BOD), and chemical oxygen demand (COD) were evaluated. Laboratory analysis revealed variations in these parameters across different locations along the river. While some parameters fell within acceptable limits according to standards, others indicated elevated pollution levels. Notably, dissolved oxygen levels were either zero or below 3 parts per million (PPM) at several sites, signalling significant water pollution. This could be attributed to high discharge from the catchment area, industrial effluents, and various drainage inputs. Furthermore, correlation analysis was conducted to understand the interrelationship between different parameters, providing insights into how changes in one parameter may affect others. This analytical approach aids in comprehending the dynamics of water quality and pollution in the Gomti River.